??>? BD?A?9 R??bjbj2DIl????????::::V ??nnnnnnnnSUUUUUU$ "tyQ?nnnnny???nn????n??n?nS?nS??????Snb ?????:??S?0',?? ?S????????bSO Yb/gSvQ(WMQu~S-Nv^(u ? ? ? ? ? ? ? ? ? ? ? y\lgV[e ~~-NvY(lWxkW(WRP[QbRP[b_bN.?O_ N\bSQ[|\0 Te 1uN2uvZT\O(u Oˆ}v(?RP[vNNTb_b'YRP[Q~ _N[NbSQ[|cv ُ1\O_vS_RvbS NNbSO_}Y/fۏLS^0Vdk bSO Y_N/fq_TMQu~Sgr~gvW,gV }0bSO Y(Antigen retrieval AR)b/g ^z(WFraenkel-conratI{N[1]N|RuirSf[xvz ~1991t^shiI{N[2]SU\0bSO Y/fcw!?0QQ0kph0QXeRGrMQu~~Sf[IHC MR(uˆ}vv?0?\ }0hb?m'`BR0_lQmTё^\vI{ OcvvbSQ[|bS'`vbS͑ef2?bbS'`_0RN[ z^vb` YǏ z0bSO Yg'Y z^0Wb` Y(W6RGrI{Ǐ z-N_c1YvbS'` OSeg:N N(Ww!?RGr NۏLIHCvYbSO_No}Yvgr~g b:NNy gHeveQece0,gevvv/fiAR-HICvSU\0^(uThQS0 ARb/g RpARb/g _lRpel. (W O~hQel,~1??01?lT(u3%H2O2;eQn'`Ǐ'l(S)irv?w!?RGr~4lQm >en(WQXeCoplin8-N0ReQARm Y4l0Qm0ё^\vm0?\ }I{ v Nv^n[(u_lpRp5b10Rla2bkRGrr^ 0 ge(WRp5RT 1R QRp5R(W,{N*N5RThKmmSOؚ^ 0RpT N_lpSQQXeCoplin8 QtS15R0GrP[~4lQmN!kT(WPBSm-Nxm5R MbۏLIHCgr?0:NNOcNvRpagN _{[`я_lp-N_v TvMOnS TNSvCoplin8 cgq N TvbSO[ N TvRpe =\Sv^zhQvRpagN0 _lMW RpǏ zY N(WN(uvQ>eeQ200mlgjx?QmPH6.0?0.1 v^v N&^ g\T[vvP[ _lRpl~?\1??lSTv~~RGrnNؚ)nQXeRGrg-N >eeQ]l~?Qm-N g\OcOZPMQu~SeǑ(u_lp-NI{Rs800W[3] _lp ?u㉻QchvVN[(u_lp(9hncvMRvxvz,^(u;S(u_lp) -Nch~~Yt10-20RSQ_lQXe8QtS[)n NQm-NSQsGr GrP[~4lQmN!k KNT(uPBSPH7.2-7.4 Qm$N!k k!k3R0 =\{ g\pe\O[4]:N~ؚ)nTQtSُNekweu0(WbN‰p 15RvQtS_{vQpt1u10YُNekweu [N gNbSO ?OMNOAR-IHCgr?_^020z6qNؚ)n0RNO)nS[~~GrcGr030S_wb_`f[vSS0 US~Rpel. ?\RGr>eeQv ggjx?Qmv[hV-N,n5up600W NRpl~?v^c~10min 0ShiI{N[2](uUS~RpelN_lRpelkIHCgr?_^ >f:y$Nyel[v Tvgr?_^0 ؚSRpel. ?ShinI{N[5]SU\N+T4lؚSnqlel egX:_y\lgV[v~~tauˆ}vMQuS^'`0\RGrޏ Tgjx?Qm>eeQؚSQ RpNuUl v^c~2min SRy_pn Rpwvc6R_͑ N~~RGr>eeQQm0RؚS?RSRy_pn;`e(W5-8R eǏSOOgr̀ofRm0sՋBRlQScOv'YYpebSO^O(uؚSnqlel ُQt^v[h?f ǑSdkelSMQGP3?04?` OIHCgrHegfsO0 ^RpARb/g ^RpARb/gSbvmS0x?lI{el0vMR ;N/fvmS vmS/fNSf[velegSbe.?O YbS0 ˆ}vvmSl.S0.05gb0.1gˆ}vvReQ100ml0.05b0.1PH7.8ve4l/lS4lnm-N nsSSb1uȏecOv0.125%vmSOv?0\RGr>eeQnvQ37oC)n{-NmS18-20R0 Àˆ}vvmSl . (u0.1mol/L HCLM?Rb0.4vÀˆ}vv? ˆ}vvmSl . \ ˆ}vvnN0.5mol/LpH7.4vTris-HCL-N Sm^:No.1 mSe20min0 bSO Yvel b sQ|0R~~bS&Tf2EQR ُ[MQu~SgrHeg g_'Yq_T0Vdk^S_9hnc N TvbSyrp Ǒ(u N TvO Yel0[gN~ހQbSYFas0Bax0Fg!I{ T~ހ(bSYLaminin0CoIVI{ R?uvmSlYtRGr08^(uvmSv?Nˆ}vvTÀˆ}vv?0N,eg ˆ}vvmSRÀˆ}vv?_ ;N(uN~ހQbSvmS Àˆ}vv?N(uN~ހbSvmS0]\O-NwSgqbSOffNc:yۏLmSvv b ُ7h['`f:_ hHegft`0 ;`KN,NؚSRpel0_lRpel:N3z[, ؚSRpelHegON_lRpelTUS~Rpel0nmvSm^[O YHegeNUOq_T PHeQ,{251;Sb?hnc4N^utʋe0t?RʋeTxvzv[E??(WbSO Yelĉ?xvzT^(uvW@x N R(uNˆ}vv? ?\ }TTmSb/g0vx?l㉴f2bSb/gTMW hb?m'`BRTriton X 100MW TX O YbSb/g [7] 0bSf2?bbSO YelY vQ-NSMW g|jx?QmO(uY Hegg}Y0MWO YbSvel/fw!?RGr1??04lmT>eeQO Ym-N (u250WvQRs2X5min _QtS[)n c8^ĉYt0vMRARǏ z]bR^(u(WBioTekhQꁨRMQu~SgrN-N0 N0AR^lav Rpc~eT:_^/f͑vq_TV }KNN ARmvPHf:y0[gN^h?WހFmbh?W8hQvbS ~ǏϑO YT ~'YRh?W8hQ OY P1850Bcl-20P-gp0Nm23 gNh?W8hQvbS~ǏϑO YOQs(WހFmQ OYP530PcNA0Ki-67I{[6]0(WO(u勹eleN[\_ [~gv$Re_N[‰0W\OQRg O[I{NSsz|v?lU_v?TRT ~ǏbSO YN N~ǏbSO Y vQgrHegf>f N T Tv3?`sf>fؚNMR[8]0d\O-N؏^laY N 10pYtT^la6qQtS0 20pYte2bkRGrr^q0 30^9hnc N TvbSO bTvbSO Yel0 40NybbSvhKmvQ)n^TeN[OcN0 50lab_`f[~gv9eS(N,~ؚ)nO YTހFmOf>fv4xOW [~ހ8hvq_T'Y OQs8h4xˆ ς(g }mgI{sa? ~v?l㉄vRGr vQ~ހFm4xOWƉmSe _ FO8h4xOW{?0 60Yg(W8^(uvQm-Nel[sbSO Y b~O YTbS[MOSu9eS S9e(uNN N8^(uvQm bRNNTBR YEDTAI{S9eUgNbSvO Y0 (uNIHCmSvv_Y @b vvy{| O(uvSm^ PH

y,2000 7 ? Kacke Ng%fIQ NNSΑI{N. X:_MQu~ހSf[grOea'`veb/gxvzS^(u.ʋeutf[Bg_.2000.7(2):147 8 ? O[ Hs~ ulI{N. bSO Y[z|v?lU_vMQu~SgrHegvq_T.ʋeutf[Bg_.2002.9(2):116 9 ?Taylor CR, Shi SR, Chalwun B,et al .Standardization and reproducibility in diagnostic immunohistochemistry.Hum.Pathol.1994;25:1107-1109 10 Shi SR,Imam SA,Yong L,et al . Antigen retrieval immunohistochemistry under the influence of PH using monoclonal antibodies.J Histochem Cytochem,1995;43:193-201 11 Suurmeljer AJH,Boon ME. Notes on the application of microwares for antigen retrieval in paraffin and plastic tissue sections.Eur.J.Morphol.1993;31:144-150 12 ?ѐ Q_N TNVf. N TbSO Ym[bS_lO Y~gvq_T.4N^N[utf[Bg_.2000;16(6):521 13 ?Shi SR,Coto RJ,Tayor CR.Antigen retrieval immunohischemistry and molecular morphology in the year 2001.Appl Immunohischem Mol Morphol 2001 Jun;9(2):107-16 14Saati T,Clamens S,Cohen-knafo E,et al.Production of monoclonal antibodies to human ER using RER.Int J Cancer 1993 Oct 21;55(4):651-4 15 Charalambous C ,Singh N, Lssacson PG .Immunohistochemical analysis of Hodgkins disease using microwave heating.J.Clin.Pathol.1993;46:1085-1088 16 Spauldlng DC.Applications of combined ISH and IHC methods for simultaneous detection of nucleic acids and proteins in pathological specimens.44th Joint Meeting of Japan Histochemical Society and the Histochemical Society.1994;July 17-23 17Almqvist PM,Mah R,Lendahl U,et al. Immunohischemical detection of nestin in pediatric brain tumors.J Histochem 2002 Feb;50(2):147-58 18 Hsu YH,Shaw CK,Chuong CM. Immunohischemical localization of DCC protein in human epithelial,neural and neuro-endocrine tissues in paraffin sections with antigen retrieval.Gaoxiang Yi Yue Za Zhi 2001 Jul;17(7):351-7 19 Shi SR,Coto RJ,Tayor . Antigen retrieval immunohischemistry used for routinely processed celloidin-embedded human temporal bone sections:standardization and development.Auris Nasus Larynx 1998 Dec;25(4):425-43 20 hi SR, Cote RJ, Taylor CR. Antigen retrieval techniques: current perspectives. J Histochem Cytochem 2001Aug; 49(8): 931-7 "$&*NPp??????????????????????? l p | ? ŹtbŮԮŮ#5B*CJOJQJ\^JaJph&5B*CJOJQJ\^JaJo(ph#5B*CJOJQJ\^JaJph&5B*CJOJQJ\^JaJo(phCJOJQJ^JaJCJOJQJ^JaJo(5CJOJQJ\^JaJo(5CJOJQJ\^JaJ5CJOJQJ\^JaJ5CJOJQJ\^JaJo(&P?????????t?!B!T!V!?????j$t$?ôôôϠϠϠ?#5B*CJOJQJ\^JaJph&5B*CJOJQJ\^JaJo(ph5CJOJQJ\^JaJo(CJOJQJ^JaJo(CJOJQJ^JaJ#5B*CJOJQJ\^JaJph&5B*CJOJQJ\^JaJo(ph3???????CJOJQJ^JB*CJOJQJ^JaJph333CJOJQJ^JaJB*CJOJQJ^Jph3335B*CJOJQJ\^Jph33301?2P. A!?"?#$%?S  iF@?F cke $1$a$$CJKHPJ_HaJmH nHsH tHA@?? ؞k=W[SOID(?????b???: @ b ???HK?0??0? 0?????0?0????0?0?0?0?0 ?? !??(?????????????#???????????????????? 89:<NOUV\]de|????????????????? ho???????????????? "(-.@AMPVYjkot~??????????????? >A????????????????????????'+058=DGHTWZabjmu????????????????  ???????????? ;<PSXkqv??????????????????    0 2 3 5 G J K N P T \ ^ k l ????????????????????    $ 9 : ?????????????????  @ C P R _ a n p ??????????/ 3 ? @ B E Y [ ????????????7 8 ] c e q ~ ????????????????? +/4:PUW]a???????????????CHJMOQ???????????? "-/49:<IM]`y{????????????????????????7T?????????? Ri?????????)?EFKLNOV`mo?????????????nuv}~???????"????????? 'Wdip??????????*27J????N[ly?????????????!()27Hn??????????? !3QZx?????()1KEHEK??$(FG??EIux????<???+2|???????? #&+.Z[bgmp??????????    ????????* + L P a d   N Q ??bhjm???jr??Z`u???ov?????????K33333ss333ss333333ss3333s33ss333s333s3s3333333s333333333333K cathy wang4D:\͑De (W Legend1200 N\MQuf[b/gDe\bSO Yb/gSvQ(WMQu~S-Nv^(u.docjm8C:\WIN98SE\Desktop\[el6eƖ\MQuf[\MQu~S\bSO Yb/gSvQ(WMQu~S-Nv^(u.doco?8 M???^`M?B*o(ph0H\??H^H`\?)?\???^`\?.\???^`\?.?\??4^?`\?)? \???^ `\?.| \??| ^| `\?.? \?? ^? `\?)?\???^`\?.o?B?w        @??y??L**??IPPL@P*PX@P.P`@UnknownG??Times New Roman5?Symbol3&? ?Arial;??[SOSimSun q??h?f Z R 1!-!),.:;?]}????    & 6"0000 0 0 00000 =@\]^?([{?  0 0 00000;[?????2?? 2QbSO Yb/gSvQ(WMQu~S-Nv^(u cathy wang cathy wang? Oh+'0???????? $0 L X dpx??#ԭ޸黯еӦs? cathy wang?athath Normal.dot? cathy wang?10hMicrosoft Word 9.0@ԭ?@g??@ ??R? ՜.+,0 hp|??? ???? ? ??JMg1 ?? #ԭ޸黯еӦ Ŀ  !"?$%&'()*+,-./0?2345678?:;<=>?@??C???Root Entry ?F???E1Table#?WordDocument2DSummaryInformation(1DocumentSummaryInformation89CompObjfObjectPool????????  ?FMicrosoft Word ĵ MSWordDocWord.Document.8?q