?? > ? B D ? A ?9 R? ? bjbj 2D I l ? ? ? ? ? ? ? ? : : : : V ? ? n n n n n n n n S U U U U U U $ " t y Q ? n n n n n y ? ? ? n n ? ? ? ? n ? ? n ? n S ? n S ? ? ? ? ? ? S n b ???? ? : ? ? S ? 0 ' , ? ? ? S ? ? ? ? ? ? ? ? bSO Yb/gSvQ(WMQu~S-Nv^(u ? ? ? ? ? ? ? ? ? ? ? y\lgV[e~~-NvY(lWxkW(WRP[QbRP[b_bN.?O_ N\bSQ[|\0Te1uN2uvZT\O(uOˆ}v(?RP[vNNTb_b'YRP[Q~_N[NbSQ[|cvُ1\O_vS_RvbS NNbSO_}Y/fۏLS^0VdkbSO Y_N/fq_TMQu~Sgr~gvW,gV }0bSO Y( A n t i g e n r e t r i e v a l A R ) b/g^z(WF r a e n k e l - c o n r a t I{N[ 1 ] N|RuirSf[xvz~1 9 9 1 t^s h i I{N[ 2 ] SU\0bSO Y/fcw!?0QQ0kph0QXeRGrMQu~~Sf[I H C MR(uˆ}vv?0?\ }0hb?m'`BR0_lQmTё^\vI{OcvvbSQ[|bS'`vbS͑ef2?bbS'`_0R N[z^vb` YǏz0bSO Y g'Yz^0Wb` Y(W6RGrI{Ǐz-N_c1YvbS'`OSeg:N N(Ww!?RGr NۏLI H C vYbSO_No}Yvgr~gb:N Ny gHeveQece0,gevvv/fiA R - H I C vSU\0^(uThQS0 A R b/g RpA R b/g _lRpel. (W O~hQel, ~1??01?lT(u3 % H 2 O 2 ;eQn'`Ǐ'l( S) irv?w!?RGr~4lQm>en(WQXeC o p l i n 8-N0ReQA R mY4l0Qm0ё^\vm0?\ }I{v Nv^n[(u_lpRp5 b1 0 Rla2bkRGrr^ 0 ge(WRp5 RT1 RQRp5 R(W,{ N*N5 RThKmmSOؚ^ 0RpTN_lpSQQXeC o p l i n 8QtS1 5 R0GrP[~4lQmN!kT(WP B S m-Nxm5 RMbۏLI H C gr?0:NNOc NvRpagN_{[`я_lp-N_vTvMOnST NSvC o p l i n 8 cgq NTvbSO[ NTvRpe=\Sv^zhQvRpagN0 _lM W RpǏzYN(WN(uvQ>eeQ2 0 0 m l gjx?QmP H 6 . 0 ?0 . 1 v^v N&^ g\T[vvP[_lRpl~?\1??lSTv~~RGrnNؚ)nQXeRGrg-N>eeQ]l~?Qm-N g\OcOZPMQu~SeǑ(u_lp-NI{Rs8 0 0 W [ 3 ] _lp ?u㉻QchvVN[(u_lp( 9hncvMRvxvz, ^(u;S(u_lp) -Nch~~Yt1 0 - 2 0 RSQ_lQXe8QtS[)nNQm-NSQsGrGrP[~4lQmN!kKNT(uP B S P H 7 . 2 - 7 . 4 Qm$N!kk!k3 R0 =\{ g\pe\O[ 4 ] :N~ؚ)nTQtSُ Nekweu0(WbNp1 5 RvQtS_{vQpt1u1 0Yُ Nekweu[N gNbSO ?OMNOA R - I H C gr?_^02 0z6qNؚ)n0RNO)nS[~~GrcGr03 0S_wb_`f[vSS0 US~Rpel. ?\RGr>eeQv ggjx?Qmv[hV-N, n5up6 0 0 W NRpl~?v^c~1 0 m i n 0S h i I{N[ 2 ] (uUS~RpelN_lRpelkI H C gr?_^>f:y$Nyel[vTvgr?_^0 ؚSRpel. ?S h i n I{N[ 5 ] SU\N+T4lؚSnqlelegX:_y\lgV[v~~t a u ˆ}vMQuS^'`0\RGrޏTgjx?Qm>eeQؚSQRpNuUlv^c~2 m i n SRy _pnRpwvc6R_͑N~~RGr>eeQQm0RؚS?RSRy _pn;`e(W5 - 8 ReǏSOOgr̀ofRm0sՋBRlQScOv'YYpebSO^O(uؚSnqlelُQt^v[h?fǑSdkelSMQGP3?04?`OI H C grHegfsO0 ^RpA R b/g ^RpA R b/gSbvmS0x?lI{el0vMR;N/fvmSvmS/fNSf[velegSbe.?O YbS0 ˆ}vvmSl. S0 . 0 5 g b0 . 1 g ˆ}vvReQ1 0 0 m l 0 . 0 5 b0 . 1 P H 7 . 8 ve4l/lS4lnm-NnsSSb1uȏecOv0 . 1 2 5 % vmSOv?0\RGr>eeQnvQ3 7 o C )n{-NmS1 8 - 2 0 R0 Àˆ}vvmSl . (u0 . 1 m o l / L H C L M?Rb0 . 4 vÀˆ}vv? ˆ}vvmSl . \ ˆ}vvnN0 . 5 m o l / L p H 7 . 4 vT r i s - H C L -NSm^:No . 1 mSe2 0 m i n 0 bSO Yvel bsQ|0R~~bS&Tf2EQRُ[MQu~SgrHeg g_'Yq_T0Vdk^S_9hnc NTvbSyrpǑ(u NTvO Yel0[gN~ހQbSYF a s 0B a x 0F g!I{ T~ހ(bSYL a m i n i n 0C o I V I{ R ?uvmSlYtRGr08^(uvmSv?Nˆ}vvTÀˆ}vv?0 N,egˆ}vvmSRÀˆ}vv?_;N(uN~ހQbSvmSÀˆ}vv?N(uN~ހbSvmS0]\O-NwSgqbSOffNc:yۏLmSvv bُ7h['`f:_hHegft`0 ;`KN, NؚSRpel0_lRpel:N3z[, ؚSRpelHegON_lRpelTUS~Rpel0nmvSm^[O YHegeNUOq_TP H